|
KMID : 0377519900150040319
|
|
Chung-Ang Journal of Medicine
1990 Volume.15 No. 4 p.319 ~ p.327
|
|
|
Separation of Component Proteins in Cystic Fluid of Taenia solium Metacestodes by Gel Filtration
|
|
Choi, Chang-Sig/최창식
Kong, Yoon/Kang, Shin-Yong/Cho, Seung-Yull/공윤/강신영/조승열
|
|
|
Abstract
|
|
|
Cystic fluid of T. solium metacestodes has been used as a diagnostic antigen for human cysticercosis. Because of cross-reactions with other parasitic infections especially with human hydatidosis sera, the nature of antigenic proteins in CF should be studied in more details. Hitherto, only a protein of 150 kDa in F, structurally similar with antiger.
B in HF´, has been known. The proteins in CF were separated into 7 fractions by filtration through Sephacryl 5-300 Superfine. Of them, fraction I, III, and IV were major frac tions. Fraction VII was considered as a degradation product and salts in eluent.
MW of proteins in each fraction at their peak point was 860 kDa in fraction I, 386 kDa´ in fraction II, 134 kDa in fraction III, 42 kDa in fraction IV, 8.5 kDa in fraction V, and 7 kDa in fraction VI. By non-denaturing disc-PAGE of CF and its fractions; main protein band in fraction III was found to be band
C protein while that in fraction IV was newly recognized band N, which dispersed between
band U and band C and stained faintly. By analysing the results of reducing and non-reducing SDS-PAGE of CF and its fractions, the 150 kDa protein in fraction III was con-firmed to be composed of 3 subunits of 15, 10, and 7 kDa. Higher molecular weight proteins in fraction I and fraction II were sub-divided into 94, 64, 39, and 26 kDa subunits. The most remarkable finding was that the protein in fraction IV showed 44-46 kDa and 21-26 kDa bands in non-reducing SDSPAGE while it showed subunits of 21, 18, 15 and 10 kDa in reducing SDS-PAGE. And 64 kDa band was ad&:tionally found in reducing SDS-PAGE of fraction IV as well. as in fraction I and fraction II. Further studies are necessary to find out the relation-ship of non-denatured proteins and their subunits especially in proteins in fraction I and fraction IV.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|